Thaum xaiv ib daim nyias nyias, daim nyias nyias thiab qhov pore loj yuav tsum tau txiav txim raws li qhov hnyav molecular, hydrophobicity, thiab lwm yam ntxwv ntawm lub hom phiaj protein, raws li zoo raws li cov kev cai ntawm downstream daim ntaub ntawv. Feem ntau cov proteins tuaj yeem siv daim nyias nyias nrog qhov pore loj ntawm 0.45 µm, thaum rau cov proteins uas tsis muaj molecular hnyav lossis peptides (kDa), daim nyias nyias uas muaj qhov pore loj ntawm 0.1 lossis 0.2 µm raug pom zoo. PVDF daim nyias nyias yog qhov kev xaiv zoo tshaj plaws rau hydrophobic proteins (xws li membrane proteins).
Txawv daim nyias nyias yuav tsum tau sib txawv pretreatment kauj ruam. PVDF daim nyias nyias yog hydrophobic heev thiab yuav tsum tau qhib los ntawm wetting nrog methanol los yog ethanol ua ntej immersion nyob rau hauv lub hloov tsis. Nitrocellulose thiab nylon daim nyias nyias feem ntau tuaj yeem sib npaug ncaj qha rau hauv qhov hloov tsis tau.
Cov txheej txheem tseem ceeb ntawm electrotransfer muaj xws li ntub electrotransfer, semi- qhuav electrotransfer, thiab qhuav electrotransfer. Electrotransfer ntub dej yog haum rau ntau yam ntawm cov protein molecular hnyav, thaum semi- qhuav electrotransfer yog sai dua tab sis haum rau me me molecules.
Cov methanol concentration nyob rau hauv qhov hloov pauv tsis cuam tshuam rau cov protein khi thiab gel shrinkage. Tam sim no, qhov hluav taws xob, thiab lub sijhawm yuav tsum tau ua kom zoo kom tsis txhob muaj cov protein nkag los ntawm daim nyias nyias. Txhawm rau tiv thaiv daim nyias nyias los ntawm kev ziab tawm hauv cov kauj ruam tom ntej, PVDF daim nyias nyias yuav tsum tau muab kaw thiab khaws cia rau hauv qhov chaw txias, qhuav.