Peb hom kev hloov pauv feem ntau siv rau kev kuaj xyuas cov protein thiab nucleic acid yog nitrocellulose daim nyias nyias, polyvinylidene fluoride (PVDF) daim nyias nyias, thiab nylon daim nyias nyias. Nitrocellulose daim nyias nyias yog ib qho uas feem ntau siv matrix hauv cov protein blotting vim lawv cov protein ntau khi affinity thiab compatibility nrog ntau yam kev tshawb nrhiav. Lub peev xwm ntawm cov protein khi ntawm nitrocellulose daim nyias nyias yog 80-100 µg / cm², thiab protein immobilization feem ntau tshwm sim los ntawm kev sib cuam tshuam ntawm hydrophobic. Nitrocellulose daim nyias nyias yog nkig thiab tawg, txwv lawv siv rau delabeling thiab relabeling.
Polyvinylidene fluoride (PVDF) daim nyias nyias muaj kev sib txuas siab rau ob qho tib si proteins thiab nucleic acids. PVDF membranes yog hydrophobic heev thiab yuav tsum tau ua ntej -wetted nrog methanol los yog ethanol ua ntej immersion nyob rau hauv kev hloov tsis. Lub peev xwm ntawm cov protein khi ntawm PVDF daim nyias nyias yog 170-200 µg / cm². Piv nrog rau nitrocellulose, PVDF daim nyias nyias yog tsawg nkig thiab tsis yooj yim, muaj zog mechanical zog thiab tshuaj stability, thiab yog haum rau Western blotting thwmsim yuav tsum tau delabeling thiab relabeling kauj ruam. Vim lawv cov hydrophobicity, PVDF daim nyias nyias yog ib qho kev xaiv rau hydrophobic proteins.
Nylon daim nyias nyias khi cov protein thiab nucleic acids los ntawm ionic, electrostatic, thiab hydrophobic kev sib tshuam. Lub peev xwm ntawm cov protein khi ntawm nylon daim nyias nyias tuaj yeem ncav cuag 480 μg / cm². Nylon daim nyias nyias muaj lub siab ntev, muab qhov zoo hauv Western blotting thwmsim xav tau cov txheej txheem delabeling thiab relabeling. Ib qho teeb meem tseem ceeb ntawm kev siv cov nylon daim nyias nyias rau blotting daim ntaub ntawv yog lub peev xwm rau tsis yog - tshwj xeeb khi nrog anions.